Molecular biology of human DDX49 - novel biochemical activities
Publication Date
July 31, 2023
Creator
Abstract
DExD-box helicases belong to superfamily II of RNA helicases and are characterised by a conserved Asp-Glu-X-Asp motif in their primary protein sequence. These helicases are often involved in pre-mRNA splicing, ribosome biogenesis and also cell cycle mediation; as such, they are implicated in the oncogenesis and proliferation of various cancer types. The overexpression of DEAD-box protein 49 (DDX49) has been associated with a favourable prognosis in cervical cancer patients, however, its structure and function has yet to be fully elucidated. We report that DDX49 is a dual function helicase and nuclease, and discuss how CRISPR-Cas9 mediated downregulation of DDX49 may affect the proliferation and viability of U2OS osteosarcoma cells.
Item Type
ethesis
Thesis Type
MRes
Supervisors
Subjects (LC)
Associated Schools / Departments
School of Life Sciences
eprints ID
72946
UoN Repository URI
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Molecular Biology of DDX49 - Novel Biochemical Activities (Corrected).pdf
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Full-text
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Examined
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3.68 MB
Format
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