Understanding the ZEB1 interactome during lymphangiogenesis
Publication Date
July 23, 2025
Creator
Abstract
Endothelial cells (ECs) constitute the crucial lining of blood and lymphatic vessels, controlling vascular integrity and function. During quiescence, ECs regulate junctional integrity and barrier stability. Recently, ZEB1 was hypothesised to contribute to endothelial quiescence. Integrating mass spectrometric and transcriptomic analyses in lymphatic ECs uncovered that ZEB1 contributes to junctional reorganization. Silencing ZEB1 significantly reduced VE-cadherin phosphorylation at Y731 and Y685 (59%±5.39, 71%±15,p<0.05, respectively), consequently compromising barrier integrity. Silencing ZEB1 downregulated YES and YAP1 suggesting that altered VE-cadherin signalling was a consequence of ZEB1 mediated-YES/YAP1 signalling. In LECs, silencing YAP1 induced cytoskeletal remodelling, and functionally compromised EC barrier phenocopying ZEB1 loss. Consistently, YAP1 loss showed dephosphorylation of Y685 and Y731.
Item Type
ethesis
Thesis Type
PhD
Subjects (LC)
Associated Schools / Departments
School of Medicine
eprints ID
80747
UoN Repository URI
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Name
AHMED,NADA,20217798,Final Thesis.pdf
Type
Full-text
Description
Examined. FINAL THESIS
Size
96.41 MB
Format
Adobe PDF
Checksum (MD5)
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