Raw data and analysis for paper "Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion"
Publication Date
July 22, 2016
Description
The two aims 1) identification of protein uniquely adsorbed to plasma etched tissue culture polystyrene using proteomics; 2) quantify and analyse pluripotent cell adherence to a range of arrayed micro environments on N-(4-hydroxyphenyl)methacryalamide. Purpose to identify novel protein pretreatments using high throughput screening. Previous publications have shown human pluripotent adherence to the plasma etched tissue culture polystyrene and N-(4-hydroxyphenyl)methacryalamide.
Collection dates:
May 2014-July 2014
External URI
Subjects (LCSH)
Subjects
Subjects (JACS)
Subjects (LC)
Divisions
University of Nottingham, UK Campus::Faculty of Science::School of Pharmacy
Data type
Word files; Tif files; Excel files
Grant Number
EP/H045384/1
Data collection method
An Ix51 IMSTAR microscope was used for all image acquisition. The IMSTAR microscope is equipped with an automated stage making it suitable for investigating large numbers of arrayed materials on a single slide. Acquisition was achieved using the in-built software; by generating a position list to image each arrayed spot or imaging an operator defined area. For the primary screening array a position list of 36 x 92 positions was generated and captured. For the secondary screening array a position list of 35 x 105 positions was generated and captured. Each position represents one polymer spot. Focus was calculated based on operator assigned landmarks on the area extremities. All images were captured using a ProgRes MF (Jenoptik) monochrome CCD digital camera.
Resource languages
English
Publisher
University of Nottingham
Date Issued
July 21, 2016
File(s)![Thumbnail Image]()
![Thumbnail Image]()
Name
Read this first (1).docx
Size
13.33 KB
Format
Microsoft Word XML
Checksum (MD5)
b92173c8c77277f97d52ed756f9932ee
Name
Secondary Screen.zip
Size
4.83 GB
Format
Unknown
Checksum (MD5)
29794860a9962f1884bd7f303b3418a9