Carbon Nanotubes for the Rapid Detection of Intracellular Hydrogen Peroxide and its Application in Bacterial Detection
Publication Date
July 22, 2019
Creator
Abstract
The aim of this work is to develop a biosensor capable of detecting bacteria through the specific detection of intracellular hydrogen peroxide. When bacteria interact with host phagocytic immune cells there is a burst of reactive oxygen species known as the respiratory burst. Traditionally this is thought to be a long and extended production of reactive oxygen species. Recent advances however, have shown that the respiratory burst can be more dynamic with a fast and rapid response to bacteria. Conventional techniques for the measurement of reactive oxygen species are limitedin their ability to differentiate between reactive oxygen species and to detect them in a time sensitive manner. In order to fulfil this need for a rapid sensor, an electrochemical nano-biosensor was developed that can interface with cells interacting with the cell cytoplasm and detect hydrogen peroxide in a time and concentration dependent manner. Such a development is a vital first step in the production of a rapid diagnostic device that will aid physicians in correctly treating bacterial infection and will be of particular use in the treatment of sepsis.
Item Type
ethesis
Thesis Type
PhD
Subjects (LC)
Associated Schools / Departments
School of Pharmacy (UK)
eprints ID
55823
UoN Repository URI
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thesis_JMH -postcomments.pdf
Type
Full-text
Description
Examined
Size
5.64 MB
Format
Adobe PDF
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