Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy
Publication Date
July 10, 2014
Creator
Abstract
This thesis is focused on the development of Raman micro-spectroscopy for label free imaging and discrimination of stem cells. The thesis is divided into six chapters. Chapter 1 gives an overview of the existing techniques used for molecular analysis of cells, with emphasis on methods that allow non-invasive label-free imaging. A literature review of the main relevant applications of Raman micro-spectroscopy for imaging cells was also included. Chapter 2 discusses the basic theoretical principles of Raman scattering and design of Raman micro-spectrometers. The practical aspects related to the design of an optimised Raman micro-spectrometer are presented in Chapter 3 along with experimental characterisation of its performance. The chapter concludes with examples of Raman spectral maps of endothelial cells. Chapter 4 and Chapter 5 present experimental results obtained by Raman micro-spectroscopy for molecular analysis of live neural and mesenchymal stem cells. In these investigations Raman spectroscopy was used to identify, image and quantify spectral markers for label-free discrimination between glial cells and their neural progeny. The potential of Raman micro-spectroscopy to measure timecourse molecular changes of individual bone nodules was demonstrated in Chapter 5. Future work and final conclusion are discussed in Chapter six of this thesis.
Item Type
ethesis
Thesis Type
PhD
Supervisors
Subjects (LC)
Associated Schools / Departments
School of Physics and Astronomy
eprints ID
14021
UoN Repository URI
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PhD_thesis_Adrian_Ghita.pdf
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Full-text
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