Secondary ion mass spectrometry analysis of pentosan polysulfate
Publication Date
March 9, 2023
Creators
Description
Pentosan polysulfate sodium (PPS) is a glycosaminoglycan mimetic that is the only current active pharmaceutical ingredient (API) for the oral treatment of interstitial cystitis. PPS is an inherently complex API due to variations in chain length, backbone structure, sulfation pattern and end group making discriminating between batch-to-batch as well as manufacturer-to-manufacturer differences difficult. In this study high throughput liquid handling and time-of-flight secondary ion mass spectrometry (ToF-SIMS) is used to enable the rapid chemical analysis (450 samples in 6 hours) of small amounts (≈150 ng) of PPS. Chemical discrimination between PPS produced by different manufacturers and, in one case, batch-to-batch variation of the same manufacturer is achieved. Analysis of samples using a 3D-OrbiSIMS instrument is used to provide confident assignment of the discriminating ions to enable structural interpretation of the variance observed between samples. Together with analysis of samples spiked with NaOAc or NaSO4, free sulphate and acetate along with sulphate pattern are implicated as key differences between manufacturers/batches. This approach is likely to be highly beneficial for the rapid quality control of PPS.
Subjects
Subjects (JACS)
Subjects (LC)
Divisions
University of Nottingham, UK Campus::Faculty of Science::School of Pharmacy
Data type
Secondary ion mass spectrometry analysis of pentosan polysulfates
Funders
Funders
University of Nottingham
Data collection method
ToF-SIMS and 3D-OrbiSIMS
Resource languages
English
Publisher
The University of Nottingham
Date Issued
April 30, 2023
Except where otherwise noted, this item's license is described as
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Name
A1_LMIG_ORBI_N.TXT
Description
3D-OrbiSIMS negative mode of PPS from manufacturer A
Size
424.53 KB
Format
Text
Checksum (MD5)
22f7e78fe80621ae9e048e1941c27af2
Name
C1_LMIG_ORBI_N.TXT
Description
3D-Orbi SIMS negative mode of PPS manufacturer C
Size
585.05 KB
Format
Text
Checksum (MD5)
28318e8ba8ce7fc2d3738ec2f8b76173