Electrochemical Analysis of Gold Nanoparticles Multifunctionalised with Cytochrome c and a Zinc Porphyrin
Publication Date
February 9, 2024
Creators
Description
Cytochrome c (Cyt c), known for its functional redox capabilities, plays a pivotal role in biological processes such as the electron transport chain and apoptosis. However, understanding how different conjugation strategies impact its structural and redox characteristics is limited. To fill this gap, we investigated the effects of conjugating Cyt c and a zinc(II) porphyrin (Zn Porph) to gold nanoparticles (AuNPs). We used circular dichroism (CD) spectroscopy to detect structural conformational changes in Cyt c upon conjugation and time-of-flight secondary ion mass spectrometry (TOF-SIMS) to identify protein orientation. Cyt c was predicted to have different orientations depending on the size of AuNPs and methods used to conjugate the protein, it was hypothesised that the orientation of Cyt c may influence the redox properties of the protein. The electrochemical properties of Cyt c were assessed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). We used DPV-based to determine the heterogeneous rate constant (k0). The results show a lower k° for conjugated Cyt c than free Cyt c, likely due to structural changes in the protein. The spatial orientation of Cyt c had minimal influence on k0, while ligand density and AuNP size had an effect. The k0 value of Zn Porph did not decrease on conjugation. Despite these changes, Cyt c and Zn Porph maintained their electrochemical capabilities after conjugation.
Collection dates:
02/01/2021 to 15/04/2022
Associate publ. DOI
Subjects
Subjects (JACS)
Subjects (LC)
Divisions
University of Nottingham, UK Campus
Data type
Excel files of CVs, DPVs, ToF-SIMS and CD
Funders
Lluisa Perez-Garcia
Frankie James Rawson
David Brian Amabilino
Grant Number
EP/R004072/1
Data collection method
Potentiastat, CD software, ToF-SIMS software
Resource languages
English
Publisher
The University of Nottingham
Date Issued
February 9, 2024
Except where otherwise noted, this item's license is described as
File(s)![Thumbnail Image]()
![Thumbnail Image]()
Name
100nm AuNP-PEG-C OX W.csv
Description
100nm AuNP-PEG-C OX W
Size
9.18 KB
Format
Comma Separated Variable (CSV)
Checksum (MD5)
9ecec0d69fdf353c4e80ba6226b578c3
Name
20nm AuNP-MUA-C Electro OX.csv
Description
20nm AuNP-MUA-C Electro OX
Size
9.03 KB
Format
Comma Separated Variable (CSV)
Checksum (MD5)
d59bb8f54146d7e2b0809389ef0470a4