Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation - Supporting data 1
Publication Date
June 4, 2015
Description
A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.
Data presented herein includes time-of-flight secondary-ion mass spectrometry (ToF-SIMS), X-ray photoelectron spectroscopy (XPS), water contact angle (WCA) measurements, fluorescence microscopy, cell electrophysiology, nuclear magnetic resonance (NMR) spectroscopy, gel permeation chromatography (GPC).
Please note that this data set is in two parts, the second part of which can be found here: http://doi.org/10.17639/nott.16
Parent projects:
'Future-Proof' Synthetic Surfaces for the Automated Manufacture of Human Pluripotent Stem Cells (EP/H045384/1)
Collection dates:
01/10/2010 - 01/06/2015
External URI
Associate publ. DOI
Subjects
Subjects (LC)
Divisions
Faculties, Schools and Departments::University of Nottingham, UK Campus::Faculty of Medicine and Health Sciences::School of Medicine
Data type
Raw data files for ToF-SIMS, XPS, WCA, Cell Microscopy, Electrophysiology, NMR and GPC datasets.
Funders
UK Regenerative Medicine Platform
British Heart Foundation
Heart Research UK
National Centre for the Replacement, Refinement and Reduction of Animals in Research
Grant Number
EP/H045384/1
Data collection method
ToF-SIMS (SurfaceLab - ION-TOF), XPS (CASA XPS), WCA, Cell Microscopy (IMSTAR), Electrophysiology, NMR (BRUKER) and GPC (Shimadzu) datasets.
Legal and ethical issues
A patent has been filed in relation to the work described herein.
Resource languages
English
Publisher
University of Nottingham
Date Issued
June 8, 2015
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DSpace data.zip
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65.54 MB
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Unknown
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