Discovery of a novel polymer for xeno-free, long-term culture of human pluripotent stem cell expansion
Publication Date
January 20, 2021
Description
Discovery of poly(TCDMDA-blend-BA) polymer substrate offers a cost-effective and xeno-free adherent culture system for human pluripotent stem cells (hPSCs) in E8 medium. This is a scalable substrate which can be readily applied to common tissue culture plasticware (TCP), whilst retaining stem cell integrity after long-term serial passage. Fulfillment of all regulatory cell culture requirements makes this a desirable expansion platform for clinical-scale, therapeutic and biomedical applications.
Data presented herein includes fluorescence microscopy of high-throughput attachment screening studies.
Parent projects:
'Future-Proof' Synthetic Surfaces for the Automated Manufacture of Human Pluripotent Stem Cells (EP/H045384/1)
Collection dates:
01/12/15 - 01/08/19
Associate publ. DOI
Related resources
Subjects (LCSH)
Subjects
Subjects (JACS)
Subjects (LC)
Divisions
University of Nottingham, UK Campus::Faculty of Medicine and Health Sciences::School of Medicine
Data type
cell microscopy, Phosphokinase array, protein adsorption surface analysis, growth curves cell analysis, integrin blocking assays
Funders
British Heart Foundation
National Centre for the Replacement, Refinement, and Reduction of Animals in Research (NC3Rs)
Grant Number
EP/N006615/1
Data collection method
Cell microscopy (IMSTAR), Operetta (Perkin Elmer), Phosphokinase R&D kit, liquid extraction surface analysis-tandem mass spectrometry (LESA-MS/MS; TriVersa Nanomate- Advion Biosciences), Cell analysis with Cedex HiRes Analyzer-the Roche Cell Counter
Legal and ethical issues
A patent has been filed in relation to the work described herein.
Resource languages
en_US
Publisher
The University of Nottingham
Date Issued
January 20, 2021
Except where otherwise noted, this item's license is described as
File(s)![Thumbnail Image]()
![Thumbnail Image]()
Name
Figure 2.zip
Size
199.4 MB
Format
Unknown
Checksum (MD5)
63070c1762e633bef50de25f9eafe38f
Name
Figure 3.zip
Description
Figure 3: Characterization of poly(TCDMDA‐blend‐BA) surface. Atomic force microscopy
Size
42.26 KB
Format
Unknown
Checksum (MD5)
b3c1b4fea4b1dbc9d72c10cdf37dd838