Immune Modulation by Design: Using Topography to Control Human Monocyte Attachment and Macrophage Differentiation
Publication Date
May 5, 2020
Creators
Description
Material topography is known to influence macrophage attachment and phenotype, providing opportunities for the rational design of ‘immune-instructive’ topographies to modulate macrophage function and thus foreign body responses to biomaterials. However, no generalisable understanding of the inter-relationship between topography and cell response exists. We therefore utilise a high throughput screening approach to investigate the relationship between topography and human monocyte-derived macrophage attachment and phenotype, using a diverse library of 2176 micropatterns generated by an algorithm. We use machine learning to successfully build a model that correlates cell attachment and phenotype with a selection of descriptors, illustrating that materials can potentially be designed to induce pro-inflammatory, anti-inflammatory or regulatory immune responses, for future application in the fight against foreign body rejection of medical devices.
Collection dates:
2018-2019
Associate publ. DOI
Subjects (LCSH)
Subjects
Subjects (JACS)
Subjects (LC)
Divisions
University of Nottingham, UK Campus::Faculty of Science::School of Pharmacy
Data type
Excel spreadsheets, GraphPad prism files, jpeg images, Notepad (readme) files
Grant Number
EP/N006615/1
Data collection method
Microscopy, XPS, Tof-SIMS, ELISA, immunofluorescent staining
Resource languages
English
Publisher
The University of Nottingham
Date Issued
May 5, 2020
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