In situ protein identification, mapping and depth profiling using 3D OrbiSIMS
Publication Date
August 28, 2020
Creators
Description
Label-free protein characterisation at surfaces requires digestion or matrix application prior to mass spectrometry. We report the assignment of undigested proteins at surfaces using secondary ion mass spectrometry (SIMS). Ballistic fragmentation induced by a gas cluster ion beam (GCIB) produces peptide fragments for subsequent OrbitrapTM analysis. We annotate 16 example proteins (up to 272 kDa) by de novo sequencing and apply the methodology to characterising a protein monolayer biochip and in situ depth profiling of proteins through human skin.
Subjects (LCSH)
Subjects
Subjects (JACS)
Subjects (LC)
Divisions
University of Nottingham, UK Campus
Data type
3D OrbiSIMS spectra, images, depth profiles
Grant Number
EP/P029868/1
EP/L01646X/1
EP/P031684/1
Data collection method
3D OrbiSIMS, individual settings for different samples described in the methods section
Resource languages
English
Publisher
The University of Nottingham
Date Issued
August 28, 2020
Except where otherwise noted, this item's license is described as
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